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Class Notes for Biochemistry at University of Regina

BIOC312 Lecture Notes - Lecture 10: Enzyme Kinetics, Enzyme, Metabolic Pathway

Lecture 10; Enzymes Reversible Enzyme Inhibition Inhibitor (I) binds to an enzyme and prevents either formation of enzyme- substrate complex (ES) or catalysis to E + 
Competitive, Non- competitive, U...

Biochemistry
BIOC312
Stefan Idziak
BIOC312 Lecture Notes - Lecture 9: Epimerase And Racemase, Stereospecificity, Binding Energy

Lecture 9; Enzymes
 Enzymes are proteins with a 3D structure which provides an active site for; 
 Binding of substrates and catalytic conversion to products Enzymes may be sing...

Biochemistry
BIOC312
Stefan Idziak
BIOC312 Lecture Notes - Lecture 8: Lipid Bilayer, Fluid Mosaic Model, Endoplasmic Reticulum

Lecture 8; Biological Membranes
 Biological Membranes Examples; Plasma membranes, Mitochondrion, Chloroplast, Transport vesicles, Nuclear envelope, Golgi apparatus, Endoplasmic reticulum, Specialised vesicles Biological...

Biochemistry
BIOC312
Stefan Idziak
BIOC312 Lecture Notes - Lecture 7: Glycosidic Bond, Fischer Projection, Anomer

Lecture 7; Carbohydrates, Nucleic Acids, Lipids
 Carbohydrates (Hydrate of carbon) have empirical formulas of (CH O) 2 n where n >= 3 Monosaccharides; One monometric unit
 Ogliosaccharide; ~2-20 monosaccha...

Biochemistry
BIOC312
Stefan Idziak
BIOC312 Lecture Notes - Lecture 5: Phosphorylation, Isomerase, Pyruvic Acid

Lecture 5; Glycoysis Glycolysis is a ubiquitous catabolic pathway that is central to metabolism. Glycolysis converts 1 glucose molecule → 2 pyruvate molecules Pyruvate can be further metabolized to; • Lactate or ...

Biochemistry
BIOC312
Gary Tompkins
BIOC312 Lecture Notes - Lecture 1: Particle Size, Antigen, Fragment Antigen-Binding

Biochemistry Part II
Lecture 1 and 2; Protein Purification Protein purification; Disruption of cells and tissues
Differentials centrifugation to isolate cellular cellular organelle
Separation of proteins on the basis of;...

Biochemistry
BIOC312
Gary Tompkins
BIOC312 Lecture Notes - Lecture 6: Thioester, Malate Dehydrogenase, Symporter

Lecture 6; Citric Acid Cycle
 • Aerobic catabolism (Oxidation reactions)
 • Intermediates used for biosynthetic reactions
 • Takes place in the mitochondria in eukaryotes or the cytosol in bacteri...

Biochemistry
BIOC312
Gary Tompkins
BIOC312 Lecture Notes - Lecture 4: Covalent Bond, Nucleoside, Coenzyme A

Lecture 4; Metabolism Metabolites, Catabolic Reactions and Anabolic Reactions Metabolism- Chemical reactions which occur within the cell
 Metabolites- Compounds which take place
 Catabolic Reactions- Reaction in whic...

Biochemistry
BIOC312
Gary Tompkins
BIOC312 Lecture Notes - Lecture 3: Christian B. Anfinsen, Dipeptide, Hydrophobic Collapse

Lecture 3 Protein Folding
 Levinthal Paradox
 Ramachandaran considered dipeptide A-B. Number of conformations of A-B is determined by rotatable bonds around bond (Φ and Ψ). For protein with 100 aa, there will be 3 = 5x...

Biochemistry
BIOC312
Gary Tompkins

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