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DNA Notes

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University of British Columbia
ANTH 200
Lynn Alden

1 DThe rate and direction of DNA synthesis is the same for the leadinglagging strands so A and B are wrongBoth require DNA separation so C is wrongStatement E doesnt make senseDNApolymerase uses the structre of the helix to choose the right bases look back at the lectures on DNA structureWhen syntehsis just startes there are not enough bases to make a helix 2 CThe centrifugal of the CsCl coupled with diffusion result in the formation of a gradient 3 EControl of the operon is at two pointsinitiation of transcription whether or not it is started and attenuation whether or not it continues4 AThis protein binds the autoinducer 5 BThe cell is still capable of repression but not capable of attenuation because it wont make a 34 loop 6 CIn order to revert a template is neededA deletion mutation would remove sequences from the template 7 DIf the error occurs during transcription only the proteins that are made by translating it may be affected depending on the nucleotide where it is etc there may not be any changeThe cell will also be transcribing this gene quite regularly since the cell has lots of ribosomes so other copies of rRNA may not be mutated 8 EYou might think that the mRNA was transcribed from an operon and each gene in the operon would have its own ribosome binding site 9 Byou have to write things out with this question 10 Cyou have to write things out with this question 11 A 12 EAll of the above could change the reading frame and and result in the formation of a stop codon which resulted in a truncated protein 13 BIf the CG ratios are different it means that the DNA sequence is differentIn order for homologous recombination to occur after DNA enters a competent cell the DNA sequences have of the donor DNA and recipient DNA need to be nearly identical 14 A 15 CTransducing phages have accidentally packaged a piece of bacterial DNA instead of a copy of the phage genomeThe key here is that the transducing particle contains bacterial DNA not phage DNA 16 DThe RE would recognize sequences in the donor DNA and cleave the DNA into smaller fragments 17 ASince the plasmid doesnt have to get be recombined with the chromosome the DNA of the donor and recipient can be different with respect to nucleotide sequenceD and E are correct statements but dont explain the question 18 EOne of the daughter cells will have the donor DNA and therefore one strand that is complementary to the donor DNA which was single strandedThe other daughter cell will have the wild type DNA 19 EA mobilizable plasmid has an oriT site that can be recognized by the nickase and the nickase gene but does not have a the tra operon
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