BIOL 111 Lecture 20: Plant Pathogens
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A 2-year-old male child was brought to the emergency room to determine why he was suddenly refusing to walk and complaining of pain in his right leg. There was no history of trauma. He had been seen by his pediatrician earlier in the day due to the presence of fever and with an upper respiratory infection; he was diagnosed with otitis media, prescribed an antibiotic, and sent home.
Physical tests in the emergency room revealed pain and tenderness of the right ankle. There was also some edema and erythema. X-ray tests of the lower extremities did not reveal any fractures or displacements. Laboratory tests were ordered on his peripheral blood and a fluid aspirate was taken from the ankle. Lab results on these samples are listed on the table below:
Peripheral Blood WBC Count | 26,000/μL |
Peripheral Blood Differential | Neutrophils 77% Lymphocytes 19% Monocytes 4% |
Erythrocyte Sedimentation Rate | Elevated |
Plasma Glucose | 96 mg/dL |
Ankle Fluid Physical Appearance | Yellow, purulent |
Ankle Fluid WBC Count | 22,000/μL |
Ankle Fluid RBC Count | 375,000/μL |
Ankle Fluid Differential | Neutrophils 95% Macrophages 5% |
Ankle Fluid Glucose | 39 mg/dL |
What is the name for the fluid collected from the patientâs ankle, and what is the procedure used to collect that fluid called?
What collection tubes should the fluid be placed into, and to which laboratory department does each tube go?
Which lab results are abnormal?
What type of disorder do you suspect causing the illness in this patient? (More than one answer may be correct)
What additional testing could be performed to prove your suspected diagnosis?
1. Helicobacter pylori, a pathogen of the stomach,produces a powerful urease. Why might urease be a useful enzyme forthis microbe?
2. T/F If two different tests can be done in MRVP medium, youshould add the methyl red (MR) and Voges-Proskauer (V-P) reagentsto the same tube.
3. After incubation, a citrate test for Escherichiacoli is green. What is the best explanation for thisresult?
4.Select the appropriate statements regarding the decarboxylasetests.
Only one type of decarboxylase is present in the medium. | |
Sterile mineral oil should be added to prevent artificialalkylation of the media�s surface. | |
The test organism is inoculated into the tube by stabbing theagar. | |
During the early stages of incubation, the medium will appearyellow; if the targeted decarboxylase is present, the medium willgradually turn purple. | |
During the early stages of incubation, the medium will appearpurple; if the targeted decarboxylase is present, the medium willgradually turn yellow. | |
The test organism is inoculated into the tube by adding a fewdrops of an isolate. | |
Proteins are not present in the test media, it is simplysupplemented with a specified amino acid. |