MICB 202 Lecture Notes - Lecture 10: Internal Ribosome Entry Site, Eukaryotic Initiation Factor, Vpg
Document Summary
Vp1, vp2, and vp3: vp4 is not visible from the surface as it is completely internal to the capsid. Immature form (non-infectious: 3 different polypeptides: vp0, vp1, vp3, 180 protein molecules. Mature form (infectious: 4 different polypeptides: vp1, vp2, vp3, vp4, 240 protein molecules. To mature, the rna genome is inserted into the capsid and cleaves the vp0 polypeptide into. Cd155 (cid:894)p(cid:396)otei(cid:374)(cid:895): host (cid:272)ell"s virus receptor i(cid:374)te(cid:396)a(cid:272)ts (cid:449)ith the (cid:862)(cid:272)a(cid:374)(cid:455)o(cid:374)(cid:863) of the vp1 of poliovirus (pvr = poliovirus receptor) Poliovirus genome: made of positive (+) sense ssrna, both ends modified, 5" e(cid:374)d: (cid:272)o(cid:448)ale(cid:374)tl(cid:455) atta(cid:272)hed s(cid:373)all, (cid:271)asi(cid:272) p(cid:396)otei(cid:374) vpg, 3" e(cid:374)d: polyadenylated. Note: the(cid:396)e is (cid:374)o 5" methylated guanine cap. The genome contains a single open reading frame (encodes a 247 kda polyprotein). Poliovirus encodes a gene for an rna dependent rna pol (rdrp). The (+) sense rna genome could be translated upon infection to synthesize this enzyme.
