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Lecture

Outline of Lecture 02

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School
Department
Biology
Course
BIOL 2050U
Professor
Peter Cheung
Semester
Fall

Description
Outline of Lecture 02 Immunoglobulins II, Genetic Diversity I. Structural basis of Ag binding A. Hypervariable regions are CDR1-3, corresponds to loops that bind Ag B. Binding pocket made of CDRs from both heavy and light chains, mostly hydrophobic interactions C. Ab’s made of linked globular domains, each with the immunoglobulin fold II. V(D)J recombination A. Genome structure: - κ (light): V-J joining, followed by single C exon - λ (light): V joins JC - Heavy: DJ joining then VDJ joining, C exon chosen by RNA splicing B. Gene rearrangements detectable by southern blot C. Molecular mechanism - RSS sequences (7/12/9 or 7/23/9 structure) are joined, 12-23 rule - RAG-1 and RAG-2 are transposases that cut RSSs - Recombination mediated by general DNA repair mechanism - DNA junction can have N region (TdT mediate
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