BCH 2333 Lecture Notes - Lecture 20: Thermal Cycler, Antimicrobial Resistance, Expression Vector

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Bound by ssdna binding proteins - binds ssdna only! Not sequence specific bc holding entire genome. Not a lot of interactions with the nucleic bases but with the sugar backbones and phosphates o. Synthesis of lagging stand dsdna at the replication fork: https://www. youtube. com/watch?v=-mtlxpgjhl0. E. coli has 2 dna polymerases: need 2 to complete the lagging strand: Dna polymerase iii (pol iii) synthesizes the leading strand and most of the lagging strand: does most of the work (main enzyme, has proof reading ability. Makes less than 1 mistake in a million. Dna polymerase i (pol i) removes rna primers and re[places them with dna: also had 5" 3" exonuclease activity o. 3" exonuclease function of dna pol i. Blue = template strand, red = okazaki fragment (p = primer) Exonuclease: starts at 5" end and cuts nucleotides out o. Pol iii is released when it gets to the next okazaki fragment.

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