BCH 4122 Lecture 16: Lecture 16 Notes_ Transition state analogues in structures of ricin and saporin
29 Mar 2018
School
Department
Course
Professor
Document Summary
Lecture 10 notes transition state analogues in structures of ricin and saporin (paper #8) M. -c. ho, m. b. sturm, s. c. almo et v. l. schramm, Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins pnas (2009) 106, 20276-20281. The activity of n-glycosidase specific rips effects the cleavage of the ribose-adenine bond of. A4324, which is situated in the conserved saracin/ricin loop of 28s ribosomal rna (rrna) The product of this reaction (a desadenylated rrna) then becomes the substrate for a lyase specific for the cleavage of the phosphodiester bond on the 3" side. A short 3" fragment (4325: and a long 5 fragment of rrna (1-4324) with a 3" hydroxyl result, puriet al. , curr. A highly toxic protein derived from the castor oil plant (ricinus communis) Acids res. (2006) 34, 697-708. diagrams of the 5"-c gaga g-3" rna tetraloop (top) and the 5"-a gtta t-3" dna tetraloop (bottom)
