BIO 1140 Lecture Notes - Lecture 14: Enzyme, Rna, Mature Messenger Rna

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magentawolf8

8 Nov 2016

School

University of Ottawa

Department

Biology

Course

BIO 1140

Professor

Caroline Petit- Turcotte

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Document Summary

Bio - lec 14 - transcription pt 1. Transcription of dna to rna : 5" to 3" Rna is oriented 5" to 3", therefore it is complementary to 3" to 5" dna strand rna polymerase ii. Reads the 3" to 5" dna strand, making the 5" to 3" complementary rna strand. Grabs all new nucleotides (not just replacing t for u) , Dna is transcribed to pre-messenger rna (extra step to mature into mrna), then it is transported out of the nucleus. Rna polymerase binds to promoter region to initiate transcription sigma units detaches, transcription continues. Sigma factor travels to attach to another incomplete rna polymerase, and initiate transcription elsewhere. At the end of coding region , rna polymerase detaches. To initiate transcription : needs proteins to bind, to recruit polymerase (tic - transcription initiation complex) Promoter region : protein binds to tata box, recruits other proteins (tata binding proteins)

Related Questions

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.