BIO 1140 Lecture Notes - Lecture 14: Stem-Loop, Tata Box, Sigma Factor

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violetseal552

28 Mar 2017

School

University of Ottawa

Department

Biology

Course

BIO 1140

Professor

Caroline Petit- Turcotte

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Document Summary

The rna stand produced is elongated in the 5" to 3" direction. The rna polymerase ii tease the 3" - 5" dna strand (the template strand) The mrna obtained is 5" - 3". Alp - what is the role of the sigma factor in transcription. A protein that recognizes the promoter region at the beginning of the gene where all the things that assemble to initiate transcription. Tbp (tata box binding protein) binds to the tata sequence on dna. Additional protein come and complete the transcription initiation complete (these include transcription factors and regulatory elements) This provides stability and allows rnapol ii to come and start transcription. Within the promoter, some proteins and transcription factors bind to speci c sequences and form the transcription initiation complex. Only occurs in eukaryotes because of complexity of dna. Rna pol ii adds 20-60 nucleotides per sec. Contrary to dna polymerase it doesn"t proofread or make corrections. Atp dependant unwinding enzyme at 3" end.

Related Questions

During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'â5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. Which of the following initially determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA?

A. the location of specific proteins (transcription factors) that bind to the DNA

B. the specific sequence of bases along the DNA strands

C. the location along the chromosome where the double-stranded DNA unwinds

D. the position of the geneâs promoter on the chromosome

E. which of the two strands of DNA carries the RNA primer

1) In Eukaryotes, repetitive sequence inclues:
A) Centromere sequences
B) Telomere sequences
C) All of the above

2) Satelit DNA:
A) Was orignally discoverd in E. Coli
B) Represents plasmid DNA sequences
C) Contains repetitive DNA sequences
D) Has the same density as main band DNA
E) All of the above

3) Inversions _____ The number of crossover offspring.
A) Decrease
B) Increase
C) Do not change
(I Believe the answer is "C" ^. can you explain why?)

4) In human genome, repetitive DNA is estimated to be ___ of the total genome.
A) .1%
B) 1%
C) 10%
D) 50%
E) 90%

5) In E. Coli, the promoter sequence:
A) Contains a short AT rich sequence
B) Binds transcription factors
C) All of the above

6) In eukaryotic transcription:
A) Transcription factors bind to the promoter and then RNA polymerase binds.
B) Transcription factors bind to RNA polymerase which then binds to the promoter
C) Transcription factors are NOT required to initiate transcription

Explanation for each would help immensely. THANK YOU in advance for your time!!!

cerisebadger105

1. The Pribnow Box is a

A. attenuator

B. enhancer

C. promoter

D. operator

2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?

A. the splicing of introns

B. addition of a poly-A at the 3' end

C. removal of introns

D. addition of a 7-methyl guanosine cap at the 5' end

3. In transcription, RNA polymerase binds at

A. a promoter

B. an operator

C. the oriC locus

D. an enhancer

4. The Central Dogma in genetics describes

A. the pattern of information flow in the cell

B. the pattern of chromosomal inheritance in populations

C. the role of mutations in disease

D. the role of promoters

5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?

A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'

B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'

C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'

D. none of the above

6. Removal of introns from RNA is by

A. primosomes

B. peptidases

C. galactosidases

D. spliceosomes

7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?

A. molecular weight

B. Svedberg units

C. map units

D. Daltons

8. During transcription, which strand is not read by the RNA polymerase?

A. coding strand

B. noncoding strand

C. template strand

D. terminator strand

9. The function of the rho protein is

A. to help terminate translation

B. to help RNA polymerase bind to the DNA

C. to help RNA polymerase find a promoter

D. to help terminate transcription

10. The unusual bases found in transfer RNAs

A. are added during transcription

B. are normal bases that have been modified after transcription

C. help the tRNAs to bind to DNA

D. ensure that the tRNA stays linear in solution

BIO 1140 Lecture Notes - Lecture 14: Stem-Loop, Tata Box, Sigma Factor

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