BIO 1140 Lecture Notes - Lecture 13: Hemolysis, Solution, Cell Membrane

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Table #1: Time in seconds needed for red blood cells to reach hemolysis in five different 5 mL solutions.
Solution
Tube 1
Tube 2
Standard
Deviation
Standard
Error
Average
Mean
Distilled
Waterd
>2
>2
0
0
2
Ureab
3
3
0
0
3
Ethylene
Glycola
16
13
1.52
0.87
14.66
Glycerola
840
840
0
0
840
Sucrosec
<1200
<1200
0
0
1200
aalcohol
bbase
csugar
dneutral
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Document Summary

Table #1: time in seconds needed for red blood cells to reach hemolysis in five different 5 ml solutions. The outer and inner sides of the membrane are made up of hydrophilic heads, while between the two layers of heads there are hydrophobic tails. Therefore, if the solute is non-polar it will be lipid-soluble and diffuse through the membrane. Although it is a large molecule, a quick hemolysis was observed with the solute urea because of its composition and lipid solubility. Solutes that are permeable or nearly as permeable as water (for example urea) do not contribute to osmotic pressure. Thus a cell will swell in a urea solution much as it will in distilled water. Subsequently the plasma membrane may rupture due to excessive internal pressure (red, n. d. ) . water on the other hand is a polar molecule but due to osmosis and aquaporins, it diffuses more rapidly than urea across the membrane.

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