BIO 3124 Lecture Notes - Lecture 7: Mycoplasma, Reverse Transcriptase, Positive And Negative Predictive Values

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November 15th lecture (slide 543 slide 570) Identifies asymptomatic individuals who may have the disease: tests for the presence of a factor associated with the disease. Diagnostic test: used to determine the presence or absence of a disease when a subject shows signs or symptoms of the disease, done after a positive screening test to establish a definitive diagnosis. Immunological: precipitation test, agglutination test, complement fixation test, elisa. Microscopy: gram stain and acid-fast stain. Identification based on metabolic characteristics: ex, oxygen requirements, carbon sources used, oxidative or fermentative metabolism, metabolic byproducts. Principle: when antibodies react with multiple epitopes on soluble antigens, there is formation of networks which generate an insoluble precipitate, precipitation reactions can take place in solution or in gels such as agar. Method: add ab against ag to detect, add a limiting concentration of complement, add igg sensitized rbcs. Dosage by elisa: used to detect the presence of antibodies or antigens, very sensitive, quantitative, quick.