BCH 2333 Lecture Notes - Lecture 17: Random Coil, Anomer, Human Genome

• School: University of Ottawa
• Department: Biochemistry
• Course: BCH 2333
• Professor: Christopher Boddy

Document Summary

Base attached to sugar at the anomeric carbon. Recall: nucleotide = base + sugar + phosphate. These are high energy phosphodiester bonds that provide e to do chemistry. Polymers of nucleotides connected by phosphodiester bonds between 5" and 3" alcohols. Recall: nucleic acids are written 5" to 3". 2 strands of dna bind together through bases. H bonds between a and t (2 h bonds) and c and g (3 h bonds) Gc content: 25 - 75% in bacteria, 39 - 46% in mammals. Bases occupy the core and sugar-phosphate chains are on the outside. Planes of bases are perpendicular to the helix axis. Each base is hydrogen bonded to the opposite strand to form a planar base pair. Helical twist of 36 per bp - rise per turn. Aromatic bases have van der waals thickness of 3. 4 - rise per base pair. Minor groove: smaller area where the phosphate backbones are beside each other.