BCH 3120 Lecture Notes - Lecture 7: Protein Phosphatase, Glycogen Phosphorylase, Phosphorylase Kinase
January 30, 2017
Regulation of Blood Sugar Levels
Regulation of Blood Glucose
Phosphorylation regulates glycogen biosynthesis/catabolism
-Glycogen phosphorylase a: glycogen catabolism
•Active when phosphorylated
-Glycogen synthase a: glycogen biosynthesis
•Active when dephosphorylated
-Both enzymes are phosphorylated by phosphorylase kinase a: activates catabolism and deactivates biosynthesis
•Kinase is active when phosphorylate by protein kinase A (PKA)
-PKA is regulated by cAMP
-Also activated by Ca2+
-Both enzymes are dephosphoryalated by phosphoprotein phosphatase 1: deactivates catabolism and activates
biosynthesis
•Phosphoprotein phosphatase 1 is inhibited by binding to phosphoprotein phosphate inhibitor-1
-Inhibitor is activated by phosphorylation by PKA
1
Document Summary
Glycogen phosphorylase a: glycogen catabolism: active when phosphorylated. Glycogen synthase a: glycogen biosynthesis: active when dephosphorylated. Both enzymes are phosphorylated by phosphorylase kinase a: activates catabolism and deactivates biosynthesis: kinase is active when phosphorylate by protein kinase a (pka) Both enzymes are dephosphoryalated by phosphoprotein phosphatase 1: deactivates catabolism and activates biosynthesis: phosphoprotein phosphatase 1 is inhibited by binding to phosphoprotein phosphate inhibitor-1. Inhibitor is activated by phosphorylation by pka. January 30, 2017 camp responds to blood glucose levels. Glucagon is released by the pancreas when [glucose] < 5 mm: increases glycogen catabolism. Will bind to receptors on liver cells, which increases camp levels. Camp will then phosphorylate phosphoprotein phosphate in order to activate glycogen catabolism. Muscle cells do not respond to glucagon, instead, they respond to epinephrine. Epinephrine will bind to receptor on muscle cells, which increases camp levels.