BCH 3120 Lecture Notes - Lecture 11: Succinic Acid, Electron Transport Chain, Acetyl-Coa
28 Apr 2018
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February 8, 2017
β-Oxidation of Fatty Acids
β-Oxidation of Fatty Acids
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Occurs in the mitochondria
Catabolism: breaks them down to form acetyl-CoA, NADH, and FADH2
-NADH and FADH2 can go directly into electron transport to produce energy
4 enzymes involved
-NB: stereochemical difference in intermediates (3-L-hydroxyacyl-CoA vs. D in biosynthesis)
Acyl-CoA Dehydrogenase
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4 different homologues in mitochondria: have different chain length selectivities
Membrane bound
Removes two hydrogen atoms to form a double bond
Uses FAD to oxidize C-C bond to form a double bond
-Similar to succinate dehydrogenase from TCA
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February 8, 2017
-FADH2 that is produced can transfer its electrons to CoQ through the electron transport chain - will make 1.5
equivalents of ATP
Enoyl-CoA Hydratase
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Water is added by enoyl-CoA hydratase (EH)
3-L-hydroxyacyl-CoA will be formed
-Note the stereochemistry
3-L Hydroxyacyl-CoA Dehydrogenase
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Oxidizes the alcohol to a ketone
Uses NAD+
-NADH that is produced can go directly to electron transport - produces 2.5 equivalents of ATP
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