BCH 3125 Lecture Notes - Lecture 15: Enzyme Catalysis, Reaction Rate, Enzyme
24 Sep 2017
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In the schemes for the catalyzed reactions presented in. The initial reaction velocity for an enzyme reaction reaches a maximum at high substrate concentration because the free enzyme can no longer regenerate at the end of each reaction cycle: at 25 c, an enzyme accelerates a reaction by a factor of 105 over the uncatalyzed reaction in water. Calculate the value of km if the forward rate constant (k1) for the e s formation is 4. 3 x 106 sec 1 m 1, the reverse rate constant (k 1) for e s dissociation is 2. 4 x 102 sec 1, and the turnover number (k2) is 1. 2 x 103 sec 1: for question #5 above, what happens to km when you make a mutation that essentially knocks out the catalytic activity of the enzyme, enzyme x lowers the activation energy of a chemical reaction by 9. 6 kjmol 1.
