BIO 3170 Lecture Notes - Lecture 16: Deoxyribonuclease, Corepressor, Gal4 Transcription Factor
6 Dec 2016
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Document Summary
Protein-protein interaction domain, the mutlipe targets the proteins have and bring together the dna binding elements. At which level do we expresse the gene. Protein-protein interaction domain important for dimerization most of these proteins act as dimers and they act with other proteins such as general transcription factors. Essentially dimer maintained as 3d structure active to bind the regulatory element. Gal4 protein has both dan binding atvity and acitvation domain. Tata bp binds to tata, regulated by gal4, which will bind to dna upstream from the tata box through the dna binding motif. Proteinproteininteraction region interacts directly with the tafs. Importatn proeins that lead to the formation of nucleation complex and eventually initiation. Part of protein is gal4, other part is lexa binding domain. Illustrate the various ways dna bp can act. Not synthesized by the cell, need to turn on gene expression for gene to see activator activity. Ligand to dna bp in order to see activity.
