BIO 3305 Lecture Notes - Lecture 1: Conformational Change, Active Transport, Aquaporin

• School: University of Ottawa
• Department: Biology
• Course: BIO 3305
• Professor: John Lewis

Document Summary

Slide 1 the problem: potential difference across the cmb, outside the cell: 0 mv. Inside the cell: -60 mv; negative relative to outside cell: can manipulate concentration inside or outside of cell (test hypothesis, find out which ions are present and then manipulate their concentrations. K+ goes out: nernst tells you the potential required to offset diff between chem gradient outside compared to chem gradient inside, at eq, the force going out balances the force going in, log function. K+ inside = 95mm, k+ out = 10mm. If k conc are equal then 0 mv: k i(cid:374) si(cid:373)ila(cid:396) k out dec(cid:396)ease co(cid:374)c g(cid:396)adie(cid:374)t so dec(cid:396)ease fo(cid:396)ce of v, so (cid:374)eed less elect(cid:396)oche(cid:373) fo(cid:396)ce, k out > k i(cid:374) positive e(cid:395) pote(cid:374)tial. Membrane transport: 3 main types, passive diffusion, facilitated diffusion, active transport, distinguished by, the direction of transport, the nature of the carriers, the role of energy. Ions normally flow through a channel: go over slides.