BIO 3305 Lecture Notes - Lecture 11: Synaptic Vesicle, Titration, Fluorescence
23 Jan 2018
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Used blocker of na/k pump and resting potential was re-achieved right away. Had little effect on resting potential (depolarized < 2mv) When have hypo effect and block it, you should see depolarizing effect. Don"t see that so the study suggests that it"s not very active. Electrogenic na/k atpase decreases excitability (increases ap threshold) Ap threshold = minimum input required to start an ap. Signal coming in as result of repeated aps that"s initiating a signalling cascade (don"t really know mechs) that results in an increased turnover (activity) in pump. K channel not regulated by mb potential but by presence of ca. Depolarization leads to ca influx leads to hyperpolarization from k channel. Cascade would not initiate and k pump would not turn on. But looking for hyperpol to see activity of pump. Block ca channels, would also block the k channels. Trying to remove hyperpol is bc of na/k pump. Block another component of pathway to be more direct.
