BPS 3101 Lecture Notes - Lecture 11: Exon, Parallelogram, Alternative Splicing
2 Nov 2016
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In our case gene is not disrupted but deleted. Promoter = small circle before bacterial resistance gene. Bar code tag = short sequence to uniquely identify a deletion mutant 20-25 nt. Yeast genes have molecular barcodes that will be different to each other although in diagram same colour. Swap out gene x and swap in antibiotic resistance. Pcr using primers with these sequences will uniquely identify mutant that is lacking gene z or use oligomer probe in southern hybridization. Collection of mutant for all of yeast genes, made individual mutants lacking gene x, gene y, etc. Swap out something and swap in antibacterial resistant gene. Cp = common priming site = uptqag barcode. F = fluorescent tag to detect hybrid on microarray. Look over gene density at end of topic 4. Top left = negative controls on chip = no signal.
