BPS 3101 Lecture Notes - Lecture 9: Restriction Site, Caveolin 1, Plasmid
2 Nov 2016
School
Department
Course
Professor
Document Summary
How to find genes within a dna seq. Check all 6 reading strands (both strands) as in q1. Note -other 5 frames have no orfsor only very short ones. Tag tga and taa occur frequently by chance (downstream of aug repeats) Aside: frames with stop codons are pooor candiadates in practice q 1. If deviation from standard code - topic 1 - can change by default. If very long gc rich seq between atc and taa,tag, tga! fortuitously. Different from pcr product bc has no introns. Results using bioinformatic tools to analyze 15 kb region of human genome. Tools to search for homologous sequences in databanks. Example of blastp search with wheat mitochodrial atp8 protein. E = stat measure that seq with his degree of similarity occur by chance. What if this search was done with nucletide instead of protein level. Homologous atp8 genes were identified only in closely-related organisms in nt search.
