BPS 3101 Lecture : Important Numbers and Abbreviations From 2010. Bonen mentioned a few important numbers to know (genome size of E.coli, etc.) so I compiled it into a review. There are also so many abbreviations that I made a list of what they all stand for

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peargoldfish669

16 Oct 2011

School

University of Ottawa

Department

Biopharmaceutical sciences

Course

BPS 3101

Professor

Linda Bonen

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Document Summary

Genomics lambda phage vector = 15 20 kb. Cos vector = 40 45 kb. Size of human genome = 3. 2 x 109 bp. Number of genes in human genome = 30 000. Size of e. coli genome = 4. 6 mbp or 4. 6 x 106 bp. Number of genes in e. coli = 4600 genes. Size of dna insert for plasmid vector = less than 10 kb. Intron length in humans ~ 200 nt to over 10 kb. Exon length in humans ~ 100 200 nt. Intron length in plants : about 100 300 nt. Intron length in yeast : 20 30 nt (for trna genes) and some ribosomal protein genes 100 500 nt. Size of genome of haemophilus = 1. 8 mbp. Number of genes in yeast = 6000. Size of poly a tail is approximately 200 nt. Microsatellites = <5 bp, clusters usually <150 bp. Lambda phage genome size = 50 kbp. Snrnas = small nuclear rna (role in splicing)

Related Questions

Please answer everything

1. Which of the following choices most accurately describes the proteins that form the linker histones of the chromatosome?

A) H2A, H2B, H3, H4

B) H2A, H1t, H5, H3

C) H5, H1a-e, H1t, H10

D) H3A, H1, H4A, H5

2. Which of the followings is not an example of randomly repeated DNA?

A) Minisatellites

B) Alphoid DNA in Centromere

C) Retrotransposons

D) Microsatellites

3) Which of the followings is not found in the human genome?

A) Long interspersed nuclear repeat

B) Short interspersed nuclear repeat

C) Insertional sequence

D) Long terminal repeat

4) Which of the following is not true?

A) Major portion of human genome is placed by repetitive sequences

B) The yeast genome is very compact

C) The higher eukaryotic organisms always have more complex genome

D) The genes make up only a small part of the human genome

5) What does the functional analysis of genome suggest the researchers about eukaryotic organisms?

A) All eukaryotic organisms have the same numbers of genes in each functional category.

B) All eukaryotic organisms have approximately the same number of genes.

C) Complex organisms have a larger portion of unknown genes

D) Complex organisms have greater numbers of genes in each functional category

E) Simpler organisms contain many fewer types of gene when compared to complex organisms

Needs help asap

_______________ are transposable elements that move within the genome by being copied and reinserted as DNA sequences, avoiding the need for reverse transcription"

	A.	Retrotransposons
	B	Retrovirus-like elements
	C	DNA transposons
	D	Satellite DNAs
	E	Long interspersed elements (LINEs)
		F.Short interspersed elements (SINEs)

The large differences observed in genomic size among eukaryotes correspond primarily to differences in

A		the amount of protein-coding genes
B		the amount of noncoding DNA
C		the biological complexity of the organisms at hand
	D	the length of the telomeres
		E the size of the centromere

The study of lncRNAs has revealed

A		that protein-coding genes are distributed homogeneously throughout the genome
B		that splicing regulates more genes than initially expected
C		that miRNAs are mostly limited to intronic sequences
D		that only a relatively small fraction of the human genome is ever transcribed
E		the existence of genomic regions coding exclusively for rRNA and tRNA
F		that extensive regions of our genome are in fact actively transcribed even though they do not contain protein-coding genes

22. What is probably the single most important distinction between bacteria and eukaryotes?

a. transcription

b. the separation of the cell's genetic material from the surrounding cytoplasm

c. the existence of ribosomes

d. RNA processing in bacteria

e. antibiotics

23. Which one does NOT characterize an open reading frame (ORF)

a. Uses six reading frames during translation

b. Contains a start codon

c. Contains a stop codon

d. Its length is in multiples of 3 nucleotides

e. It should have the number of codons for a protein or on average ~250 codons in length.

24. Nested intronic genes lie within the non-coding intronic region of a larger gene, and occur in the human genome.

a. True

b. False

25. How would you characterize this statement: EF-Tu will hydrolyze GTP only if the codon:anticodon & tRNA:amino acid matchings are correct

a. Transcription; initiation steps

b. Transcription; termination steps

c. Translation: initiation steps

d. Tanslation: elongation steps

e. Spliceosome

26. Regarding the transcription unit architecture of the Escherichia coli genome, which one is FALSE?

a. Each of the 4,661 transcription units comprised an average of 1.1 modular units.

b. 3,010 transcription units (~65%) are monocistronic.

c. 1,652 transcription units (35%) contain more than one ORF (polycistronic)

d. The largest transcription unit is equivalent to 16 ORFs with numerous introns.

e. 398 transcription units (~9%) are nested within each other in opposite transcriptional orientation

27. Regarding RNA interference (RNAi), which one is FALSE?

a. It is a common and conserved mechanism in eukaryotic cells.

b. Originally discovered in bacteria and plant viruses.

c. A form of primitive immunity to protect the genome.

d. Pre microRNA (miRNA) genes have been found in introns.

e. Recent research shows that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

28. Aminoacyl-tRNA synthetases

a. Recognize the 3âUTR

b. The genes for these enzymes fall into the family of inducible genes

c. The enzymes are imported into the nucleus

d. Covalently attaches an amino acid to the appropriate tRNA. This takes place in the cytoplasm.

e. Forms the peptide bond during protein synthesis

29. What is the process by which complementary RNAs are made from a DNA template?

a. translation

b. transcription

c. protein synthesis

d. transduction

e. transliteration

cyanbee225

BPS 3101 Lecture : Important Numbers and Abbreviations From 2010. Bonen mentioned a few important numbers to know (genome size of E.coli, etc.) so I compiled it into a review. There are also so many abbreviations that I made a list of what they all stand for

Document Summary

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