CHM 4139 Lecture Notes - Lecture 4: Reduction Potential, Molybdenum, Pyridoxal Phosphate
Document Summary
Chm 4139- lecture 4: factors that affect enzyme activities (cont"d: small molecules (includes metal ions) Inhibitors and allosteric regulators can increase and/or decrease the activity of enzymes by blocking access to the active site or by inducing structural changes in the protein. Inhibitors and allosteric regulators are not required for the reaction to work. In their presence, they affect their rate of reaction. Cofactors and coenzymes: they are not inhibitors or allosteric regulators. Cofactor: organic molecule or metal ion that is required by an enzyme for its activity. A cofactor binds to its associated protein (apoenzyme), which is functionally inactive, to form the active enzyme(holoenzyme). Will transfer things to the product that will stay on it. Cofactor binds to the enzyme and stays there. It is dissociable from the enzyme: it binds to the enzyme at the beginning of the catalytic cycle and leaves the active site after each cycle.