BIO206H5 Lecture Notes - Lecture 9: Adenine, Intron, Spliceosome
Document Summary
Mediator allows for variability in inputs: different spatial and temp. patterns expressed. C-terminal domain of rna polymerase i: plays role in regulating when gene is going to be expressed. Contains a variety of different enzymes important for the complex. Closed complex: all gtfs along with rna pol ii. Gtfiib help them to be kept separate and not re-anneal. C-terminal domain has a repeating a. a sequence (7 a. as that repeat up to 52 times in humans: y, s, p, t, s, p, s : phosphate on 5th serine. It is the pole for a variety of protein enzymes that participate in the process of rna being formed. The 5" end is chemically modified: 7-methylguanosine ca: binds to a particular location (#2 position) which allows for association into 5". Sets of reactions that occur: gtp is substrate, and so is growing rna molecule. Capping is necessary for maturation of rna molecule.
