BIO206H5 Lecture Notes - Lecture 7: Primary Transcript, Covalent Bond, Transcription Preinitiation Complex
Document Summary
Bio206 transcription of a prokaryotic gene ii. Sigma factor allows them to identify where the protein is located on the dna. Core enzyme contains catalytic activity of the enzyme beta subunit catalyzes. It is the sigma factor that is the regulatory component and recognizes the promoter. Alpha recognizes the upstream element in dna. Beta has the polymerase activity which includes chain initiation and elongation. Beta prime is involved in binding dna and stabilizes the structure on the template of. Omega is a molecular chaperone stabilizes the structure of the core enzyme keeping them all glued together. Sigma recruit rna polymerase complex recognizes and binds to specific promoter regions of a prokaryotic gene. When protein binds, it has directionality it points in one direction and pointing in that direction defines how transcription is going to occur. The beta subunit has been removed base pairs are pointing inwards and structure cannot be transcribed.
