BIO206H5 Lecture 13: Bio206 Lecture 22 October 2014

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Met (initiator trna molecule) idiffers from regular trnamet but met is not trnai formylated. Stage 2: formation of 43s initiation complex includes the following: eif-1a, eif-3, 40s ribosome, mrna, trnai. Add e infront of it initiation complex for eukaryotes small ribosomal subunit is bound with initiator trna. Mrna have 5" cap and poly- a tail. Complex wobbles down to recognize kozak sequence. Initiation complex is positioned on aug of kozak sequence. To add large 60s subunit the initiation factors separate. Gtp energizes this process by binding to eif and the hydrolysis is what drives the reactions to forward direction. With 3 stages charged trna in both a and p site different mechanisms that pro and euk have mrna aligned on small subunit. Elongation sequential addition of amino acids with hydrolysis of bond to form peptide bond. This is cayalyzed in large sub unit of ribosomal rna.

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