BIO206H5 Lecture 18: Bio 206 Lecture October 29
Document Summary
Replication bubble has 2 ends called replication forks synthesis is bidirectional as it moves in both directions of the replication bubble. Replication is asymmetric and semi-discontinuous leading strand synthesises continuously lagging strand synthesises discontinuously (okazaki fragments) Checks base pairs and able to replace mistakes. Eukaryotic chromosome is linear while bacterial is circular. Therefore telomeres are required completion of dna replication. Dna polymerase creates 3" to 5" phosphodiester bond proof reading feature allows dna pol to use 3"-5" exo nucleic capability to remove mistake and add correct bp therefore dna pol has two catalytic. Dna pol only makes mistake 1 in 1 million. Capability energy that comes for adding nucleotide comes from the hydrolyse of atp to adp. Multiple proteins are able to form phosphodiester bonds and only a few which break phosphodiester bonds. E. coli dna replication replication begins at an origin of replication a unique region of dna only.
