BIO206H5 Lecture Notes - Lecture 7: Coding Strand, Royal International Air Tattoo, Transcription Factor Ii D
Document Summary
Bio206 lecture oct 1, 2014 transcription of eukaryotic. Distal promoters: gc and caat boxes all these dna regional elements are protein binding sites. Gc box (-200 - -70) upstream houses a transcription factor called sp1, member of larger protein family called sp/klf. Made from 3 zinc finger motifs: interact at gc box. This loop structure can occupy many conformations (doesn"t have a rigid structure) There are several his and cys which interact with zn2+ which makes it a stable structure. Able to control activity by sp1 protein is by removing the zinc able to bind more efficiently. Single protein regulate protein in many different ways. Other regions of dna attract proteins which may. Mediators chromatin remodelling complexes histone remodelling complexes about 85 proteins required. Eubacteria: core rna polymerase requires sigma factor to recognize the -10 and -25 regions of the promoter. Eukaryotes: core rna polymerase ii also requires help to recognize the promoter:
