BIO206H5 Lecture Notes - Lecture 7: Lac Repressor, Permease, Lac Operon
Document Summary
There are many rules, but it"s best to go over the storyline prove this. Semi-conservative: can grow bacteria at different mediums using heavy & light n isotopes to. Dna gyrase - stability and helicase activity; equivalent to topoisomerase. Open groove is for dnab and dnac to bind. Through t-connectors: attach the 2 cores to the gamma complex. Epsilon: exonuclease activity (1 will promote, remove) Theta: add nucleotide to growing dna structure. 2 sides: for the two sides of replication form. Dna polymerase needs rna primer to begin polymerase activity to initiate adding nucleotides. Primase - lays out directionality for dna pol in addition to adding primers. No ribonuclease - rna would be part of daughter strands. Telomeres (added by telomerase) no longer present during cell division. We want to only produce proteins necessary at the time. Allow bacteria to respond to environmental change. Lactose incduces expression of a specific gene.
