BIO315H5 Lecture Notes - Lecture 5: Nuclear Localization Sequence, Nuclear Membrane, Nuclear Transport

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25 Sep 2017
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It consists of an inner and outer membrane. These two are continuous, but they have distinct proteins compositions. The inner nuclear membrane consists of proteins with binding sites for chromosomes and nuclear lamina. The nuclear lamina is a protein meshwork that acts as structural support for the nuclear envelope, an anchoring site for chromosomes, and an anchoring site for the cytoplasmic skeleton via protein complexes. The outer nuclear membrane is continuous with the er and is also studded with ribosomes. There are around 30 different protein types that reflect a high degree of internal symmetry. They are also highly similar (likely evolved through duplication). Each nucleoporin is present in multiple copes resulting in 500-1000 proteins complex. Can transport up to 1000 macromolecules/sec, and can transport bidirectionally simultaneously. Npcs have aqueous passages, and small water-soluble molecules can diffuse in these passively. Npcs: what are the two types of signals in active transport: nuclear localization signal (nls, nuclear export signal (nes)

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