BIO372H5 Lecture Notes - Dna Supercoil, Plasmid, Dna Gyrase

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21 Apr 2012
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MOLECULAR BIOLOGY
BIO372S
February 3, 2011
First mid-term examination
(one hour)
Read each question carefully and put your answers on the exam page
only in the spaces indicated.
Good luck!
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Name: page 2 of 6 pages
1 (10 points). You are given a
sample of double-stranded DNA
containing the following fragment
lengths: 100, 316, 1000, 3162, and
10000 bp. The 1% agarose gel is run
so that the smallest fragment appears
at 9 cm from the well and the largest
3 cm from the well. Show the precise
positions of the other fragments.
2 (5 points). Define "processivity" in DNA replication in no more than
12 words.
3 (5 points). In whole genome sequencing, define "contig" and
"scaffold" in no more than 20 words in total.
well
10,000 bp
100
bp
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