BIO373H5 Lecture Notes - Lecture 21: Sybr Green I, Serial Dilution, Acridine Orange
Document Summary
Net phytoplankton greater than 60nm, can be caught in a net. The organisms within the circle is basically the original food web. Nannoplankton phytoplankton which is smaller than 60microns. Mucus net makers flow through here assumed to be dead end, not really part of the food web. Dissolved organic material functional composition of material based on scale, anything smaller than an average cell. Energy isn"t exclusively just moving though the classic food chain and there are numerous levels of energy flow within the food chain and among the smaller organisms. But to be adhered to other organisms. Did try to use dyes and stains which may not have been very efficient: cholodny method complicated filtration system, bacterial estimations based on cultivation methods gave much lower estimates and direct count gave higher estimates. Varied on the basis of the method. Acridine orange epifluorescence technique epifluorescence look up. Sybr green binds specifically to ds dna.
