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Lecture 12

BIO204 Lecture 12

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University of Toronto Mississauga
Sanja Hinic- Frlog

12 October-17-13 12:08 PM Cellular respirationand fermentation are differentmethods of producingATP • Cellular respiration - Combination of glycolysis, Krebs cycle, and electron transportchain • Cellular respiration cannotoccur without oxygen as electron acceptor • Fermentation occurs in absence of oxygen and an ETC! it replacesoxygen as the electron acceptor • Fermentation allows glycolysis to continue (with e.g. ethanol or lactic acid as by-products) Cellular respiration and fermentation are different methods of producing ATP • Exampleof fermentation in humans ○ Lactic acid (anaerobicrespiration, sore muscles) • Exampleof fermentation in yeast ○ Ethanol (beer)- germs also enjoy wine so be careful  white part is the yeast cell still continuing fermentation ○ Biofuel (corn,sugar cane) • Exampleof fermentation in plants ○ Ethanol (Anaerobicrespiration in flooded root systems, e.g. poplar) Oxidative Phosphorylation vs. Fermentation • Oxidative Phosphorylation ○ Oxygen is most effective e- acceptor because of high electronegativity  large differencein potential energy between NADH and O2 electrons  generates a large proton-motiveforce for ATP production • Fermentation ○ Without O2 as an e- acceptor, cells cannot generate the large potential energy difference ○ Does not provideas much ATP as aerobic respiration • Fermentation ○ pyruvate or its derivative, rather than oxygen, accepts electrons from NADH ○ converts NADH back to NAD+, this allows glycolysis to continue to produce ATP via substrate-levelphosphorylation • Lactic acid fermentation ○ pyruvate produced by glycolysis accepts electrons from NADH. Lactate and NAD+ are produced ○ Pyruvate directly accepts electron as terminal electron acceptor • Alcohol fermentation ○ pyruvate is enzymatically converted to acetaldehydeand CO2. ○ acetaldehyde accepts electrons from NADH ○ ethanol and NAD+ are produced Fermentation RegeneratesNAD+
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