Class Notes (808,126)
Canada (493,084)
Biology (2,099)
BIO206H5 (179)
Lecture 20

BIO206 Lecture 20/22.pdf

21 Pages
Unlock Document

University of Toronto Mississauga
George S Espie

Lecture 20 22November1313520 PMsignal sequence is required for transport into the nucleusThe sequence is generally 12 positively charged areas lysine or arginineREFER TO TABLE IN YOUR TEXTinitial interaction with the pores requires aid of NUCLEAR IMPORT RECEPTORS these bind and then interact with the fibrils directing the protein into the porethe pore opens just enough to allow the complex of protein and NIR to enter and then closesGTP hydrolysis is required for this change but the exact mechanism is still unknownthe NIR dissociates in the nucleus and is transported back out of a nuclear pore to the cytoplasm recycledNLS Localization is in the body of the protein Signal Sequences Book Notes Page 1 this diagram is in your textbook and you can see the schematic shows the various stepsprotein binds the complex is transported through then they separate and the NUCLEAR IMPORT RECEPTOR is recycled back out to the cytosol to collect another proteinNote that in the nuclear to cytosol direction you dont seem to need a NIR equivalentFULLY FOLDED proteins and ASSEMBLED ribosomal subunits are tranported in order for proteins to enter the other organelles they must be UNFOLDED as we will see nextReleasing of the protein is a catalyzed reactionRAN a member of GTP binding proteinIt has higher affinity for the binding site than the transport protein Book Notes Page 2
More Less

Related notes for BIO206H5

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.