BIO314H5 Lecture Notes - Lecture 8: Hydrogen Bond, Electrophoresis, B Cell

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16 Apr 2016
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High % gel smaller pore size which means can separate smaller fragments: vice versa, low % gel has larger pores. Buffer: if to use with pcr or cloning or to check interaction with other products etc. , never use buffer with borate: so no tbe only used for analytical examination, otherwise for other procedures tae is used. Lac operon: codes for transcription of -galactosidase, which converts lactose to galactose and glucose, lac y permease and lac a - trasnacetylase. Expression vectors vectors such as plasmids or viruses which are designed for protein expression in cells. 1st march 1, 2016: bacteriophage, controlling genes in the virus, very effective, comes with primer sites which you can use to check how much of the insert went in and etc. Based on voltage potential fragmentation size exclusion. Monoclonal antibodies reliable, predictable interactions with the target antigen.