BIO328H5 Lecture Notes - Lecture 7: Nature Reviews Genetics, D. T. Suzuki, Western Honey Bee
Document Summary
Last lecture: to bee or not to bee foraging. A 191:987-994: gene expression profiles to describe the suite of genes that differ between nurses and foragers: Foraging- like would be described by predictor genes that are upregulated in foragers and down regulated in nurses. Nurse-like would be described by predictor genes that are upregulated in nurses and down regulated in foragers: whitfield et al. Identify and characterize the behaviour of interest (nurse vs forager bee behaviour) Nominate a candidate gene based on literature, database searches etc : (the drosophila melanogaster foraging gene) Clone the candidate gene in your organism: (amfor from apis mellifera bees) Expression analysis and adjustments of gene expression (qrt-pcr, and treatment with activator of gene expression 8-br-cgmp showed amfor is important for the switch between nurse and forager behaviour) Nominate a candidate gene from literature or database searches. 2005 trends in ecology and evolution 20: 96-104. Foraging is a food-related behaviour: fly larvae live in their food.
