BIO370Y5 Lecture Notes - Lecture 9: Polyarteritis Nodosa, Syphilis, Infective Endocarditis
Document Summary
Diagnostic tests utilize antibodies that detect specific antigens for a unique disease. They are: quick, sensitive, have low false positive and negatives. We will be doing latex bead agglutination: specifically testing rheumatoid factors (rf, they recognize the teritiary structure of igg fc fragments, the presence is a good indicator of arthritis and other auto-immune disorders. Latex agglutination slide test for the determination of rheumatoid factor in serum. Rapet (rapid particle enhanced technology) rf is used for qualitative and semi-quantitative detection of rf to detect rheumatoid arthritis. Rf are antibodies which go after the teritiary structure of igg fc. Positive = course agglutination against clear background (above 20 iu/ml) Negative = smooth agglutination against clear background (below 20 iu/ml) The titre of the serum is the reciprocal of the highest dilution that exhibits a positive reaction. drying effect can lead to false positives (reaction time greater than 2 minz)
