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Lecture

BIO372 - Lecture 1.pdf

5 Pages
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Department
Biology
Course Code
BIO372H5
Professor
Gordon Anderson

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Description
Lecture 1 January-09-14 10:08 AM Why study molecular biology? - Studying the scheme, why things are the way they are - More of a problem-based course Lectures and articles are posted online: http://www.erin.utoronto.ca/~w3bio372/ 1859 - Charles Darwin: A lot of molecular patterns are strongly influenced by natural selection Change variation or sequence 1869 - DNA - no idea of the function yet 1927 - Muller - huge discovery 1931 - There is crossing over between chromosomes,and recombinationoccurs Jumping beans 1944 - a huge discovery,biochemistryis linked to the genes 1958 - Semi conservativeDNA - another classic experiment 1961 - mRNA 1996 - Many investigators - eukaryotic genome - very expensive, but now is cheaper 1996 - Many investigators - eukaryotic genome - very expensive, but now is cheaper Electrophoretic mobility of DNA fragments of various sizes DNA is applied to gel, apply a current (there is a voltage) positiveterminal is down, DNA is negatively charged, the speed is relative to the size, we can only measure down to 50 (anything smaller we can't measure) This is logarithmic scale There is variation because of error in measurement Electrophoretic mobility of DNA fragments of various sizes The electroph
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