BIO372H5 Lecture Notes - Lecture 14: Radioactive Tracer, Dna Supercoil, Reagent
Document Summary
Load # of polymerases, nick the strand to relieve the strain, then ligate nick back. We can measure amount of supercoil (it will migrate faster in the gel than relaxed) We examine three different ways from initiation to elongation (hypotheses): Scrunching - proved to be true (polymerase doesn"t change) Fret alex - we are not going to make a mistake since that alone will decrease energy transfer and it will confound results. Sds linearized the amino acid chain and cover it to have equally negative charge - however, if you take sds, the protein is not likely to get back the original configuration. It not easy to form the 3d structure of protein. Urea is less harsh and will retain the structure of protein (i. e. rnase is very robust, it retains its function) There are different possible combinations of reconstitution (rifampacin-resistant at the right). As long as you have all the subunits.
