BIO372H5 Lecture Notes - Dna Supercoil, Plasmid, Dna Gyrase
Document Summary
Read each question carefully and put your answers on the exam page only in the spaces indicated. You are given a sample of double-stranded dna containing the following fragment lengths: 100, 316, 1000, 3162, and. The 1% agarose gel is run so that the smallest fragment appears at 9 cm from the well and the largest. Show the precise positions of the other fragments. Define processivity in dna replication in no more than. scaffold in no more than 20 words in total. page 3 of 6 pages. You make an equal mixture (by weight) of: i) a 200 bp segment of dna from a pcr reaction, ii) pbr322 plasmid dna, and iii) the unique fraction of agaricus bisporus dna. Dnas were previously sheared to a uniform size of 200 bp. The dna mixture was thermally denatured and then placed in standard conditions allowing renaturation. Draw a plausible renaturation (cot) curve. page 4 of 6 pages.
