CHM110H5 Lecture Notes - Lecture 6: Geostationary Operational Environmental Satellite, Type B Cipher Machine, Paper Chromatography

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Preparation of the chromatography paper, found in the chm110h5f course manual: the unknown solution was obtained from the ta, using a different capillary tube for each solution, one drop of the five metal cations, mixture of the five cations, and the unknown solution were applied to the chromatogram along the ticks marked on the paper accordingly, the sample spots on the chromatogram were allowed to dry, steps 4 5 were repeated twice, the chromatogram was rolled into a cylinder with the sample spots facing outward and connected with a piece of tape approximately 1. 5cm long leaving a gap between the ends of the paper of about 0. 5cm, the plastic wrap was removed from the developing chamber, the chromatogram was placed into the developing chamber, the developing chamber was re sealed with the plastic wrap, the chromatogram was allowed to sit in the developing chamber and the results were observed and recorded as the solvent moved up the paper.

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