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Lecture 5

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University of Toronto Scarborough
Horatio Morgan

Lecture 5  Meranda fruit can be toxic; some species avoids them and some prefer them; some can detect them by smell but others cannot  D. sechellia has fewer OR (olfactory receptors) and GR (gustatory receptors) genes than D. melanogaster; look at lecture slides  GR is important for detection of octanoic acids  Obp56e decreases the avoidance of meranda fruits; loss of its function means that they avoid it  However the difference was only like 15% in avoidance  Your going to have to know these taste receptors  Mammals prefer sweet food; it is calorie dense  Knowing that a fruit is ripe tells us that there are possibly less toxins in it; like mangos that are not completely ripe taste bitterish and riped ones taste good and sweet  Different mice desired different amounts of “saccharine”; some preferred someone didn’t  After crossing these mice, they found that the generation after were mostly tasters; this showed that its most likely dominant  The numbers from the table in the lecture show us that it’s a simple loci gene; meaning that it was a simple either dominant or recessive;  After like 15 years later, the exact location of the gene was found and they were able to either remove that specific gene and put in the taster gene; so in effect, either you could change a non taster to a taster and a taster to a non taster; the gene is called T1R3  So anyways, they used sucrose as the control and also saccharin; saccharin is an artificial sweetner; so basically, if the flies only desire something which is sweet they would have affinity for both; however, if their affinity is more to sucrose then that means they are getting nutritional value out of it; so basically if they never went to the saccharin, that would mean that they have these sweet things because of the nutrition,
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