BIOA01H3 Lecture Notes - Lecture 7: Nuclear Membrane, Cell Plate, Microfilament

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BIOA01H3 Full Course Notes
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BIOA01H3 Full Course Notes
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Dna is heated to split dna into single strands. Making specific primers that anneal to both 5" ends of the desired region. Taq polymerase added along with all other nucleotides. Lower temperature enough to allow dna synthesis to occur. Temperature lowered again and primers bind to their complementary sequences and taq polymerase is used again the synthesize dna until temp is raised again to end the second cycle. After 14 cycles, about 1 million copies are made. Normal dna polymerase would be destroyed in the high heat needed to split dna: taq polymerase had to be obtained. A heat-resistant polymerase found in organisms in hot springs. Other big challenge is primers: need to know the 5" end complementary bases. In g2, chromosomes are packaged in such a way that allow them to still undergo transcription and translation: solenoid fibers are in looped domains but can"t really see them in g2.

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