BIOA01H3 Lecture Notes - Lecture 26: Cas9, Forskolin, Crispr

• School: UTSC
• Department: Biological Sciences
• Course: BIOA01H3
• Professor: Karolyn Keir

Document Summary

Watson&crick: find out ladder-like double helix dna struc w/ help of rosalind. Franklin (use x ray to find out dna struc) , (francis crick, james watson,) Maurice wilkins (@ growing strand) 3 oh attacks phosphate bond of coming nucleotide (3p -> 2p = pyrophosphate)-> initiate synthesis reaction by dna polymerase. Semiconservative - new dna duplex = 1 old parental strand + 1 new daughter strand. + 1 n14 x2 + 2 n14 x2 (if xsemi: 1st round = 2 n15 + 2 n14 w/ same intensity -> 2nd round = same but. N15 w/ low intensity & n14 w/ high intensity. Replication direction: 5 -> 3 (top strand elongates l -> r => top & bottom: opp direction) Rna primase bai dai 1 rna primer -> dna polymerase extends rna primer - > a diff dna polymerase removes & replaces primer w/ dna -> dna ligase join 2 okazaki fragments.