BIOA01H3 Lecture Notes - Lecture 34: Endoplasmic Reticulum, Aminoacylation, Inosine
13 May 2018
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Department
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BIOA01
LEC 34 Translation
Nov 26, 2015
→ Translating
- from language of nucleotides into language of amino acids that
form polypeptides
- mRNA directs synthesis of polypeptides
- first amino acid is always Met-_> methionine
→tRNA
- transfer rnas
- single stranded
- bring specific amino acids to ribosome
- cloverleaf shape → has anticodon sequence at bottom end
- contains Inosine→ allows other codons to combine
→ Wobble Hypothesis
- 61 different sense codons do not require 61 different tRNAs
- first two nucleotides of codon or anticodon MUST match exactly
→ Aminoacylation
- adds aa to tRNA
→ Ribosomes
- ribonucleic protein particles
- made up of RNA and robisomal protein
- work together to make up two sub units of ribosomes
- in eukaryotes float around in cytosol some stuck on outside of
endoplasmic reticulum→appears rough = ROUGH endoplasmic
reticulum
- creates one small and one large sub unit
different sites
- A site→ aminoacyl cite
- P site→ peptidyl site (polypeptide chain is formed here)
- E site→ exit site → where the chain exits when complete
- codons are read by anticodons
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BIOA01H3 Full Course Notes
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Document Summary
Translating from language of nucleotides into language of amino acids that form polypeptides. Mrna directs synthesis of polypeptides first amino acid is always met-_> methionine. Cloverleaf shape has anticodon sequence at bottom end. Contains inosine allows other codons to combine. 61 different sense codons do not require 61 different trnas first two nucleotides of codon or anticodon must match exactly. Made up of rna and robisomal protein. Work together to make up two sub units of ribosomes endoplasmic reticulum appears (cid:494)rough(cid:495) = rough endoplasmic in eukaryotes float around in cytosol some stuck on outside of reticulum. Creates one small and one large sub unit different sites. P site peptidyl site (polypeptide chain is formed here) E site exit site where the chain exits when complete. Starts when initiator trna (one that brings met) is stuck on to p site find mrna and find aug from 5(cid:495) cap find aug and stops there. Does the anticodon match exactly with codon.
