BIOA01H3 Lecture Notes - Lecture 2: X-Ray Crystallography, Dna Replication, Ethidium Bromide

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14 Aug 2018
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BIOA01H3 Full Course Notes
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Bases must be complementary due to hydrogen bonding to maximize stability in the dna molecule. Happens between electronegative atoms present in one nitrogenous base which makes hydrogen bond to a electronegative atom in the adjacent base. Adenine and thymine bond with 2 hydrogen bonds. Guanine and cytosine bond with 3 hydrogen bonds. %a = %t and %g = %c. Hydrogen bonding and base stacking contribute to the stability of dna. Allows bases to stay away from water, hydrophobic ring structure of. Hydrogen bonds are weak but together they are strong. Interaction between bases in same strand is called base stacking. Stability force occurs because non-polar flat surfaces tend to group away from h20 and stack as tightly as possible. Backbone and base stacking causes the helical structure of dna, phosphates come closer, but they do not like it because they are negatively charged. Dna in cells must be compacted because its too big to fit in the cell.

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