BIOA01H3 Lecture Notes - Lecture 31: Auxotrophy, Arginine, Citrulline

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12 Jun 2016
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Beadle and tatum grew nutritional mutants (auxotrophs) of neurospora on a minimal medium supplemented with a single amino acid such as arginine (arg). They are working on neurospora, some type of mold. They"re looking at mutations that are a problem in these cells. We"re looking at the phenotypic inability to grow. Some mutants can"t grow on certain food; they need something to be added to it. They need supplements, in this case it"s amino acids. They need a specific type of food added into their medium in order for them to grow. So, auxotrophas are ones that need something special added into their diet. They hypothesized that each auxotrophic strain had a defect in a gene that codes for an enzyme needed to synthesize a particular amino acid (one gene one enzyme hypothesis). The mutations occurring in those mutants are changes that resulted in a particular enzyme being functioning properly.

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