BIOB10H3 Lecture 4: BIOB10 NOTES
Document Summary
Studying cells in controlled and vitro systems. Used to treat: juvenile diabetes, alzheimers, (cid:708)(cid:720)(cid:737)(cid:730)(cid:728)(cid:733)(cid:738)(cid:734)(cid:733)"(cid:738, spinal cord injuries. Take a somatic cell from a sick patient. Take out the nucleus from the cell. Grow the embryonic stem cells in culture. Add media, hormones, and factors to differentiate the cells (to make whatever tissue to replenish the embryonic stem cells) eg. liver cells, muscle cells, nerve cells, blood cells, etc. Obtaining embryonic stem cells: difficult, limited numbers, controversial. (cid:758)m(cid:720)(cid:730)(cid:724)(cid:759) (cid:738)(cid:739)(cid:724)(cid:732) (cid:722)(cid:724)(cid:731)(cid:731)(cid:738) (cid:728)(cid:733) (cid:741)(cid:728)(cid:739)(cid:737)(cid:734: (cid:758)(cid:696)(cid:724)(cid:723)(cid:728)(cid:725)(cid:725)(cid:724)(cid:737)(cid:724)(cid:733)(cid:739)(cid:728)(cid:720)(cid:739)(cid:728)(cid:733)(cid:726)(cid:759) (cid:721)(cid:720)(cid:722)(cid:730) (cid:739)(cid:734) (cid:740)(cid:733)(cid:738)(cid:735)(cid:724)(cid:722)(cid:728)(cid:720)(cid:731)(cid:728)(cid:745)(cid:724)(cid:723) (cid:738)(cid:739)(cid:724)(cid:732) (cid:722)(cid:724)(cid:731)(cid:731, ips cell induced pluripotent stem cell. Biob10: eg. dynamic motility of mitochondria, mitotic chromosomes. The longer the wavelength the harder to see. The shorter the wavelength the clearer: bright- field light microscope. Can see some sort of structure: fix cells, stain cells, mount on slides. Makes transparent things visible by causing light rays to interfere with one another: differential interference contrast (dic) Both are for small, unstained specimens and increasing contrast.