๐Ÿท๏ธ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION โ†’
Log in
HomeClass Notes1,200,000CA670,000

BIOB10H3 Lecture 4: BIOB10 NOTES

68 views35 pages
School
UTSC
Department
Biological Sciences
Course
BIOB10H3
Professor
Rene Harrison

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Studying cells in controlled and vitro systems. Used to treat: juvenile diabetes, alzheimers, (cid:708)(cid:720)(cid:737)(cid:730)(cid:728)(cid:733)(cid:738)(cid:734)(cid:733)"(cid:738, spinal cord injuries. Take a somatic cell from a sick patient. Take out the nucleus from the cell. Grow the embryonic stem cells in culture. Add media, hormones, and factors to differentiate the cells (to make whatever tissue to replenish the embryonic stem cells) eg. liver cells, muscle cells, nerve cells, blood cells, etc. Obtaining embryonic stem cells: difficult, limited numbers, controversial. (cid:758)m(cid:720)(cid:730)(cid:724)(cid:759) (cid:738)(cid:739)(cid:724)(cid:732) (cid:722)(cid:724)(cid:731)(cid:731)(cid:738) (cid:728)(cid:733) (cid:741)(cid:728)(cid:739)(cid:737)(cid:734: (cid:758)(cid:696)(cid:724)(cid:723)(cid:728)(cid:725)(cid:725)(cid:724)(cid:737)(cid:724)(cid:733)(cid:739)(cid:728)(cid:720)(cid:739)(cid:728)(cid:733)(cid:726)(cid:759) (cid:721)(cid:720)(cid:722)(cid:730) (cid:739)(cid:734) (cid:740)(cid:733)(cid:738)(cid:735)(cid:724)(cid:722)(cid:728)(cid:720)(cid:731)(cid:728)(cid:745)(cid:724)(cid:723) (cid:738)(cid:739)(cid:724)(cid:732) (cid:722)(cid:724)(cid:731)(cid:731, ips cell induced pluripotent stem cell. Biob10: eg. dynamic motility of mitochondria, mitotic chromosomes. The longer the wavelength the harder to see. The shorter the wavelength the clearer: bright- field light microscope. Can see some sort of structure: fix cells, stain cells, mount on slides. Makes transparent things visible by causing light rays to interfere with one another: differential interference contrast (dic) Both are for small, unstained specimens and increasing contrast.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

BIOB10H3 Lecture Notes - Lecture 3: Antigen, Scanning Electron Microscope, Fusion Protein
orangefly323
BIOB10H3 Lecture Notes - Lecture 3: Stem-Cell Therapy, Embryonic Stem Cell, Knockout Mouse
corallocust714
BIOB10H3 Lecture Notes - Lecture 3: Glycocalyx, Insulin, Blastocyst
turquoisepug293

Related Questions

The second experiment is described in the following abstract:

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

What does this tell you about the nature of the changes that take place during the process of differentiation? How would you think that the reactivation of these genes might act?