BIOB10H3 Lecture Notes - Lecture 4: Endomembrane System, Clathrin, Lysosome

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28 Oct 2017
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Vesicle budding involves: donor compartment vesicle will form here. The vesicle: membrane enclosed dynamic cellular organelle that is involved in selective transport of proteins/materials to other compartments: recipient compartment: vesicle will fuse w/ compartment and deliver its contents. Budding vesicles have coat of soluble (cytosolic) proteins that assemble on the membranes of donor compartment. Can mechanically force membrane to curve and form a budding vesicle. Can bind to specific transmembrane proteins receptors that in turn bind specific soluble components/proteins cargo to be recruited into budding vesicle: assemble on cytosolic face of membranes and induce vesicle budding. Discovered in em as (cid:862)fuzzy(cid:863) ele(cid:272)tro(cid:374) de(cid:374)se outer layer. Cop proteins (coat proteins) cop i & cop ii. Cop ii (cid:448)esi(cid:272)les: tra(cid:374)sport protei(cid:374)s (cid:862)for(cid:449)ard(cid:863) fro(cid:373) e to golgi: anterograde transport. Cop i (cid:448)esi(cid:272)les: tra(cid:374)sport protei(cid:374)s (cid:862)(cid:271)a(cid:272)k(cid:449)ards(cid:863) fro(cid:373) golgi to e a(cid:374)d (cid:449)ithi(cid:374) golgi fro(cid:373) tgn to (cid:272)is-golgi: retrograde transport. Clathrin coated vesicles: transport proteins to lysosomes and vacuoles.

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