BIOB10H3 Lecture Notes - Lecture 12: Elution, Cytosol, Small Interfering Rna
9 Dec 2017
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Ash freeze and there is some order collect the images pick particles process data reconstruct and segment map to build model this way we can see di erent con gurations of the protein and take all the data in average. Take two proteins that can get close together and give them gfp variants. One is the donor and one is the acceptor donor uorescence will go down and acceptor uorescence will go up if fret occurs. List all the steps in doing a fret experiment. Protein localization: make gfp fusion protein use immuno uorescence (need to make antibody rst) inject mouse with puri ed protein of interest to make ab. Interacting partners loss of function analyses gene knock out sirna technology. Interacting partners pulling out your friends and hopefully you go along with them given the right conditions you can co-pull out proteins.
