BIOB10H3 Lecture Notes - Lecture 3: Stem-Cell Therapy, Embryonic Stem Cell, Knockout Mouse
21 May 2018
School
Department
Course
Professor
Lecture 3: Studying Cells/ER & Golgi
Cell Biology Techniques: Cell Culture
Cell Culture:
Studying cells in controlled, in vitro system
Grow in plastic flasks in media
Why use cultured cells in research?
Cultured cells can be obtained in large quantity
Most cultures contain only a single type of cell
Wide variety of different cells can be grown in culture
HeLa Cells
Cell Culture
Why use cultured cells in research?
Many different cellular activities can be studied Chance to study cell differentiation
Cultured cells respond to treatment with drugs, hormones, growth factors & other active
substances
Stem Cell Therapy➔ pluripotent
To treat: juvenile diabetes, alzheimer’s, parkinson’s, spinal cord injuries, etc.
find more resources at oneclass.com
find more resources at oneclass.com
Stem Cells
1) Obtain embryonic stem cells
- difficult, limited numbers, controversial
2) ‘Create’ stem cells in vitro
Induce/ reprogram differentiated cells to ‘dedifferentiate’ back to unspecialized stem cell
Express certain embryonic transcription factors
iPS cell
find more resources at oneclass.com
find more resources at oneclass.com
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Cultured cells can be obtained in large quantity. Most cultures contain only a single type of cell. Wide variety of different cells can be grown in culture. Many different cellular activities can be studied chance to study cell differentiation. Cultured cells respond to treatment with drugs, hormones, growth factors & other active substances. To treat: juvenile diabetes, alzheimer"s, parkinson"s, spinal cord injuries, etc. Difficult, limited numbers, controversial: create" stem cells in vitro. Induce/ reprogram differentiated cells to dedifferentiate" back to unspecialized stem cell. H&e (stain) of sections from gene knock-out mouse. For small, unstained specimens like living cells. Useful in examination of intracellular components in cells (fixed or living) at relatively good resolution. Resolution: the extent to which fine detail in specimen can be discriminated or resolved. Depends on the wavelength of light eg. dynamic motility of mitochondria, mitotic chromosomes. Uses accelerated electrons as a light source provides much greater resolution. Cells/tissues must be fixed (killed), embedded & manually sectioned.
